부산대학교 도서관

Salmonella sp. specific gene fragment 3335471 was used to establish a PCR-based assay for rapid detection of Salmonella sp. To establish the PCR assay, internal amplification control (IAC) was constructed using the composite primer method; the optimal concentration was determined to be 365 copies/μL. Storage of freeze-dried PCR solutions at –20°C for 3 months had no effect on detection results. Tests of artificially contaminated milk showed that Salmonellacholeraesuis could be detected in 8 h when inoculated at 7 colony forming units (CFU)/10 mL. Next, a quantitative real-time PCR (RT-PCR) for detection of Salmonella sp. was developed. The assays showed high specificity for 13 Salmonella strains and 16 non-Salmonella strains. The optimal concentration of the IAC was 425 copies/μL for RT-PCR. Using S. choleraesuis as the target strain, the detection sensitivity of genome was 1.23 fg/μL and the limit of detection was 1–4 CFU/10 mL in artificially contaminated milk with 6 h of non-selective enrichment. This study established highly efficient, sensitive PCR and RT-PCR systems, providing effective approaches for the rapid detection of Salmonella sp. in the food industry. [ABSTRACT FROM AUTHOR]