학술논문
CLOCK inhibits the proliferation of porcine ovarian granulosa cells by targeting ASB9.
Document Type
Article
Author
Source
Subject
*GRANULOSA cells
*MOLECULAR clock
*GENE expression
*BIOLOGICAL rhythms
*BIOLOGICAL systems
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Language
ISSN
1674-9782
Abstract
Background: Clock circadian regulator (CLOCK) is a core factor of the mammalian biological clock system in regulating female fertility and ovarian physiology. However, CLOCK's specific function and molecular mechanism in porcine granulosa cells (GCs) remain unclear. In this study, we focused on CLOCK's effects on GC proliferation. Results: CLOCK significantly inhibited cell proliferation in porcine GCs. CLOCK decreased the expression of cell cycle-related genes, including CCNB1, CCNE1, and CDK4 at the mRNA and protein levels. CDKN1A levels were upregulated by CLOCK. ASB9 is a newly-identified target of CLOCK that inhibits GC proliferation; CLOCK binds to the E-box element in the ASB9 promoter. Conclusions: These findings suggest that CLOCK inhibits the proliferation of porcine ovarian GCs by increasing ASB9 level. [ABSTRACT FROM AUTHOR]