학술논문
Cross-sectional study of Treponema pallidum PCR in diagnosis of primary and secondary syphilis.
Document Type
Article
Author
Source
Subject
*SYPHILIS
*CROSS-sectional method
*POLYMERASE chain reaction
*MEDICAL care
*HIV
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Language
ISSN
0011-9059
Abstract
Background Syphilis remains a major challenge and a complex diagnosis. We aim to evaluate the role of polymerase chain reaction ( PCR) in Treponema pallidum (Tp) detection in various types of biological samples in the diagnosis of early syphilis. Methods We conducted a cross-sectional study including all attendees of the STI clinic with clinical suspicion of early syphilis. One or more specimens for the detection of Tp by PCR testing were collected. Results The overall sensitivity of Tp PCR test was 82.61% (95% CI: 68.6-92.2%). Tp PCR test had sensitivity of 84.6% (95% CI: 54.6-98.1%) in primary syphilis cases and 81.8% (95% CI: 64.5-93%) in secondary syphilis cases. PCR test performance was independent of HIV status. Conclusion Tp PCR test is a fast and reliable method for the detection of Tp in skin lesions of early syphilis, and it is a powerful tool in clinical settings. [ABSTRACT FROM AUTHOR]