학술논문
The interplay of viral loads, clinical presentation, and serological responses in SARS-CoV-2 – Results from a prospective cohort of outpatient COVID-19 cases.
Document Type
Article
Author
Puchinger, Kerstin; Castelletti, Noemi; Rubio-Acero, Raquel; Geldmacher, Christof; Eser, Tabea M.; Deák, Flora; Paunovic, Ivana; Bakuli, Abhishek; Saathoff, Elmar; von Meyer, Alexander; Markgraf, Alisa; Falk, Philine; Reich, Jakob; Riess, Friedrich; Girl, Philipp; Müller, Katharina; Radon, Katja; Guggenbuehl Noller, Jessica Michelle; Wölfel, Roman; Hoelscher, Michael
Source
Subject
*PANDEMICS
*COVID-19 pandemic
*SARS-CoV-2
*DISEASE risk factors
*SYMPTOMS
*VIRAL load
*DISEASE progression
*COVID-19
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Language
ISSN
0042-6822
Abstract
Risk factors for disease progression and severity of SARS-CoV-2 infections require an understanding of acute and long-term virological and immunological dynamics. Fifty-one RT-PCR positive COVID-19 outpatients were recruited between May and December 2020 in Munich, Germany, and followed up at multiple defined timepoints for up to one year. RT-PCR and viral culture were performed and seroresponses measured. Participants were classified applying the WHO clinical progression scale. Short symptom to test time (median 5.0 days; p = 0.0016) and high viral loads (VL; median maximum VL: 3∙108 copies/mL; p = 0.0015) were indicative for viral culture positivity. Participants with WHO grade 3 at baseline had significantly higher VLs compared to those with WHO 1 and 2 (p = 0.01). VLs dropped fast within 1 week of symptom onset. Maximum VLs were positively correlated with the magnitude of Ro-N-Ig seroresponse (p = 0.022). Our results describe the dynamics of VLs and antibodies to SARS-CoV-2 in mild to moderate cases that can support public health measures during the ongoing global pandemic. • SARS-CoV-2 viral loads drop fast within one week of symptom onset. • Positive viral culture results have high viral loads and low symptom to test time. • Maximum viral loads positively correlate with the magnitude of Ro-N-Ig assay. • Maximum viral loads significantly differ between WHO groups. [ABSTRACT FROM AUTHOR]