부산대학교 도서관

Objective Systemic administration of kainic acid ( KA) is a widely used procedure utilized to develop a model of temporal lobe epilepsy ( TLE). Despite its ability to induce status epilepticus ( SE) in vivo, KA applied to in vitro preparations induces only interictal-like activity and/or isolated ictal discharges. The possibility that extravasation of the serum protein albumin from the vascular compartment enhances KA-induced brain excitability is investigated here. Methods Epileptiform activity was induced by arterial perfusion of 6 μ m KA in the in vitro isolated guinea pig brain preparation. Simultaneous field potential recordings were carried out bilaterally from limbic ( CA1, dentate gyrus [ DG], and entorhinal cortex) and extralimbic regions (piriform cortex and neocortex). Blood-brain barrier ( BBB) breakdown associated with KA-induced epileptiform activity was assessed by parenchymal leakage of intravascular fluorescein-isothiocyanate albumin. Seizure-induced brain inflammation was evaluated by western blot analysis of interleukin ( IL)-1β expression in brain tissue. Results KA infusion caused synchronized activity at 15-30 Hz in limbic (but not extralimbic) cortical areas, associated with a brief, single seizure-like event. A second bolus of KA, 60 min after the induction of the first ictal event, did not further enhance excitability. Perfusion of serum albumin between the two administrations of KA enhanced epileptiform discharges and allowed a recurrent ictal event during the second KA infusion. Significance Our data show that arterial KA administration selectively alters the synchronization of limbic networks. However, KA is not sufficient to generate recurrent seizures unless serum albumin is co-perfused during KA administration. These findings suggest a role of serum albumin in facilitating acute seizure generation. [ABSTRACT FROM AUTHOR]