부산대학교 도서관

Determination of the cellular origin of lymphoid malignancies is important for classification and management of the disease. Along with flow cytometry, immunohistochemistry is indispensable in immunophenotypic characterization of lymphomas. There are classic B-lymphocyte (B-cell) markers that have been used for identification of normal and neoplastic B-lymphocytes, such as CD markers, Kappa, Lambda, and the anti-apoptotic protein Bcl-2. Recently, a number of B-cell transcription factors have been useful in labeling B cells. Here we show a panel of rabbit monoclonal antibodies (RabMAbs) in our EP ClonesTM series detecting human normal and neoplastic B cells, including the classic B-cell markers CD19, CD20, CD21, CD23, CD79a, Kappa, Lambda, Bcl-2, and the B-cell transcription factors BOB.1, PU.1, PAX5, FOXP1, MUM1, and c-Myc. These antibodies were developed by utilizing the RabMAb® technology. All the RabMAbs to B-cell markers show high specificity and sensitivity in recognizing B-cell-specific proteins with clear background. The RabMAbs against B-cell transcription factors provide a novel approach for the analysis of lymphoid malignancies. All the antibodies are ideal for immunohistochemical detection of target proteins in formalin-fixed, paraffin-embedded (FFPE) tissues. [ABSTRACT FROM AUTHOR]