학술논문
Targeting sodium/iodide symporter gene expression for estrogen-regulated imaging and therapy in breast cancer.
Document Type
Article
Author
Source
Subject
*SODIUM iodide
*GENE expression
*GENE therapy
*CANCER treatment
*BREAST cancer
*STEROID hormones
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Language
ISSN
0929-1903
Abstract
Expression of the sodium iodide symporter (hNIS) has been detected in breast cancer tissue, but frequently, not at the levels necessary to mediate 131I accumulation. Transducing the hNIS gene into breast cancer cells with adenovirus could be a tractable strategy to render breast cancer susceptible to radioiodide therapy. We constructed the replication-incompetent virus, AdSERE, in which an estrogen-responsive promoter directs the expression of hNIS. In vitro, we demonstrate that AdSERE mediates hNIS expression and iodide uptake in ER+ breast cancer cells. In vivo, we show that AdSERE-infected ER+ tumors can be imaged due to tracer accumulation; in addition, AdSERE in combination with therapeutic doses of 131I suppresses tumor growth.Cancer Gene Therapy (2008) 15, 465–473; doi:10.1038/cgt.2008.6; published online 18 April 2008 [ABSTRACT FROM AUTHOR]