학술논문
LUNG TISSUE STORAGE: OPTIMIZING CONDITIONS FOR FUTURE USE IN MOLECULAR RESEARCH.
Document Type
Article
Author
Source
Subject
*OLIGONUCLEOTIDES
*TISSUES
*RNA
*NUCLEIC acids
*ELECTROPHORESIS
*POLYMERASE chain reaction
*EDUCATION
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Language
ISSN
0190-2148
Abstract
The quality of tissue studied impacts greatly on oligonucleotide microarray results, emphasizing the importance of harvesting techniques. The analyzed RNA extracted from human lung samples preserved via 4 different storage conditions (RNAlater, phosphate-buffered saline, TRIzol, liquid nitrogen). RNA was assessed by denaturing gel electrophoresis, Agilent bioanalysis, real-time polymerase chain reaction (PCR), and Test3 Affymetrix chip hybridization. Results revealed better quality RNA from RNAlater samples on gel electrophoresis and bioanalysis. RNAlater samples also showed greater yield (r18s via PCR P < .05) and resulted in better Test3 chips hybridization (p < .05), suggesting RNAlater was superior at preserving lung tissue nucleic acid. [ABSTRACT FROM AUTHOR]