학술논문
Protective immunity against H5N1 influenza virus by a single dose vaccination with virus-like particles
Document Type
Article
Author
Source
Subject
*VACCINATION
*INTRAMUSCULAR injections
*IMMUNE response
*INFLUENZA A virus, H5N1 subtype
*HEMAGGLUTININ
*RECOMBINANT viruses
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Language
ISSN
0042-6822
Abstract
Abstract: We generated influenza virus-like particles (VLPs) containing the wild type (WT) H5 hemagglutinin (HA) from A/Viet Nam/1203/04 virus or a mutant H5 HA with a deletion of the multibasic cleavage motif. VLPs containing mutant H5 HA were found to be as immunogenic as VLPs containing WT HA. A single intramuscular vaccination with either type of H5 VLPs provided complete protection against lethal challenge. In contrast, the recombinant H5 HA vaccine was less immunogenic and vaccination even with a 5 fold higher dose did not induce protective immunity. VLP vaccines were superior to the recombinant HA in inducing T helper type 1 immune responses, hemagglutination inhibition titers, and antibody secreting cells, which significantly contribute to inducing protective immunity after a single dose vaccination. This study provides insights into the potential mechanisms of improved immunogenicity by H5 VLP vaccines as an approach to improve the protective efficacy against potential pandemic viruses. [ABSTRACT FROM AUTHOR]