부산대학교 도서관

Abstract Ciliary beating frequency (CBF) was investigated in ciliated nasal epithelial cells (cMNECs) isolated from mice using video microscopy equipped with a high-speed camera. In cMNECs, a spontaneous CBF oscillation was observed. The CBF oscillation was abolished by BAPTA-AM but not by Ca2+-free solution. The addition of thapsigargin, which depletes Ca2+ from internal stores, also abolished CBF oscillation. Moreover, the intracellular Ca2+ concentration [Ca2+] i , spontaneously oscillated even with the Ca2+-free solution. Moreover, 2APB (an inhibitor of the IP 3 receptor) abolished CBF oscillation in cMNECs. Overall, these findings suggest that the CBF oscillation in cMNECs is triggered by the release of Ca2+ from the IP 3 -sensitive internal stores. Moreover, IBMX, an inhibitor of phosphodiesterase, did not affect CBF oscillation in cMNECs, although it slightly increased CBF. These results suggest that CBF oscillations were induced by [Ca2+] i oscillation controlled via the release of Ca2+ from IP 3 -sensitive stores, rather than via cAMP accumulation. CBF oscillation possibly plays a crucial role in maintaining an efficient mucociliary clearance in the nasal epithelia. Highlights • Spontaneous CBF oscillation was found in ciliated mouse nasal epithelial cells. • The CBF oscillation was caused by the [Ca2+]i oscillation. • The [Ca2+]i oscillations were generated by the periodic Ca2+ release from internal stores via interactions between IP 3 and IP 3 receptor. • The CBF oscillation may play a crucial role for maintaining an efficient mucociliary clearance in nasal epithelia. [ABSTRACT FROM AUTHOR]