학술논문
Time-dependent cell-state selection identifies transiently expressed genes regulating ILC2 activation.
Document Type
Article
Author
Tanaka, Yumiko; Yamagishi, Mai; Motomura, Yasutaka; Kamatani, Takashi; Oguchi, Yusuke; Suzuki, Nobutake; Kiniwa, Tsuyoshi; Kabata, Hiroki; Irie, Misato; Tsunoda, Tatsuhiko; Miya, Fuyuki; Goda, Keisuke; Ohara, Osamu; Funatsu, Takashi; Fukunaga, Koichi; Moro, Kazuyo; Uemura, Sotaro; Shirasaki, Yoshitaka
Source
Subject
*INNATE lymphoid cells
*CELL imaging
*GENES
*CELL populations
*RNA sequencing
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Language
ISSN
2399-3642
Abstract
The decision of whether cells are activated or not is controlled through dynamic intracellular molecular networks. However, the low population of cells during the transition state of activation renders the analysis of the transcriptome of this state technically challenging. To address this issue, we have developed the Time-Dependent Cell-State Selection (TDCSS) technique, which employs live-cell imaging of secretion activity to detect an index of the transition state, followed by the simultaneous recovery of indexed cells for subsequent transcriptome analysis. In this study, we used the TDCSS technique to investigate the transition state of group 2 innate lymphoid cells (ILC2s) activation, which is indexed by the onset of interleukin (IL)-13 secretion. The TDCSS approach allowed us to identify time-dependent genes, including transiently induced genes (TIGs). Our findings of IL4 and MIR155HG as TIGs have shown a regulatory function in ILC2s activation. Time-Dependent Cell-State Selection combines live cell imaging and single-cell RNA sequencing to characterize and analyse ILC2s during their activation, revealing a set of genes that are transitionally upregulated (TIGs) in ILC2s during this phase. [ABSTRACT FROM AUTHOR]