학술논문
Near-isogenic lines for resistance to brown planthopper with the genetic background of Indica Group elite rice (Oryza sativa L.) variety ‘IR64’.
Document Type
Article
Source
Subject
*NILAPARVATA lugens
*RICE
*INSECT pests
*ANTIBIOSIS
*
*
*
Language
ISSN
1344-7610
Abstract
The brown planthopper (BPH), Nilaparvata lugens Stål, is an insect pest that severely damages rice (Oryza sativa L.) in Asia, causing huge yield loss. Use of resistant variety is a cost-effective and eco-friendly strat‐ egy for maintaining BPH populations below the economic injury level. However, current BPH populations have been changed to virulence against resistant varieties. In this study, to estimate effective combinations among eight BPH resistance genes (BPH32, BPH17-ptb, BPH20, BPH17, BPH3, BPH25, BPH26 and qBPH6), eight near-isogenic lines with the genetic background of an Indica Group rice variety ‘IR64’ (IR64- NIL) were developed using marker-assisted selection. The genome recoveries of these NILs ranged from 89.3% to 98.8% and agronomic traits of them were similar to those of ‘IR64’. In modified seed box screening test, resistance level of IR64-NILs was higher than that of ‘IR64’. In antibiosis test, high adult mortalities of BPH (from 56.0% to 97.0%) were observed among NILs, in comparison with that of ‘IR64’. Among IR64- NILs, the line carrying BPH17 showed the highest resistance level at all tests. Thus, these IR64-NILs with multiple BPH resistance genes could be valuable breeding lines for enhancing resistance levels by gene pyra‐ miding and multiline variety. [ABSTRACT FROM AUTHOR]