학술논문
Megapixel digital PCR.
Document Type
Article
Author
Source
Subject
*MICROFLUIDICS
*DIAGNOSTIC use of polymerase chain reaction
*SURFACE tension
*DNA replication
*CHROMOSOMES
*
*
*
*
Language
ISSN
1548-7091
Abstract
We present a microfluidic 'megapixel' digital PCR device that uses surface tension-based sample partitioning and dehydration control to enable high-fidelity single DNA molecule amplification in 1,000,000 reactors of picoliter volume with densities up to 440,000 reactors cm?2. This device achieves a dynamic range of 107, single-nucleotide-variant detection below one copy per 100,000 wild-type sequences and the discrimination of a 1% difference in chromosome copy number. [ABSTRACT FROM AUTHOR]