학술논문
An essential role for tripeptidyl peptidase in the generation of an MHC class I epitope.
Document Type
Article
Author
Seifert, Ulrike; Maranon, Concepcion; Shmueli, Ayelet; Desoutter, Jean-Francois; Wesoloski, Lisa; Janek, Katharina; Henklein, Peter; Diescher, Susanne; Andrieu, Muriel; de la Salle, Henri; Weinschenk, Toni; Schild, Hansjorg; Laderach, Diego; Galy, Anne; Haas, Gaby; Kloetzel, Peter-M.; Reiss, Yuval; Hosmalin, Anne
Source
Subject
*PEPTIDES
*MAJOR histocompatibility complex
*DENDRITIC cells
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Language
ISSN
1529-2908
Abstract
Most of the peptides presented by major histocompatibility complex (MHC) class I molecules require processing by proteasomes. Tripeptidyl peptidase II (TPPII), an aminopeptidase with endoproteolytic activity, may also have a role in antigen processing. Here, we analyzed the processing and presentation of the immunodominant human immunodeficiency virus epitope HIV-Nef(73-82) in human dendritic cells. We found that inhibition of proteasome activity did not impair Nef(73-82) epitope presentation. In contrast, specific inhibition of TPPII led to a reduction of Nef(73-82) epitope presentation. We propose that TPPII can act in combination with or independent of the proteasome system and can generate epitopes that evade generation by the proteasome-system. [ABSTRACT FROM AUTHOR]