학술논문
Cutting Edge: The RNA-Binding Protein Ewing Sarcoma Is a Novel Modulator of Lymphotoxin b Receptor Signaling.
Document Type
Article
Author
Source
Subject
*EWING'S sarcoma
*RNA-binding proteins
*TUMOR necrosis factors
*LYMPHOID tissue
*MASS spectrometry
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Language
ISSN
0022-1767
Abstract
Lymphotoxin ß receptor (LTßR) signaling is crucial for lymphoid tissue organogenesis and immune homeostasis. To identify novel regulatory mechanisms for signaling, we implemented a two-step screen that uses coexpression analysis of human fibroblasts undergoing LTbR stimulation and affinity-purification mass spectrometry for the LTbR signaling protein TNFRassociated factor 3 (TRAF3). We identify Ewing sarcoma (EWS) protein as a novel LTßR signaling component that associates with TRß3 but not with TNFR-associated factor 2 (TRAF2). The EWS:TRAF3 complex forms under unligated conditions that are disrupted following activation of the LTbR. We conclude that EWS limits expression of proinflammatory molecules, GM-CSF, and ERK-2, promoting immune homeostasis. [ABSTRACT FROM AUTHOR]