학술논문
Effect of genotype and medium culture content on microspore-derived embryo formation in Chinese cabbage ( Brassica rapa ssp. chinensis) cv. Lastochka.
Document Type
Article
Author
Source
Subject
*CHINESE cabbage
*BRASSICA
*MICROSPOREA
*GENOTYPES
*PLANT embryology
*HAPLOIDY
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Language
ISSN
1062-3590
Abstract
The influence of different factors on microspore embryogenesis in Chinese cabbage ( B. rapa ssp. chinensis) was studied. A genotype dependence for embryo formation was observed. The majority of embryos and plants were obtained from microspores isolated from flower buds (2-2.9 mm in length) and cultured in the NLN liquid medium with 13% sucrose (w/v) supplemented with 24-epibrassinolide and 1% activated charcoal. Embryos cultured on the 1/2 Murashige-Skoog culture medium with 2% sucrose (w/v), 0.1 mg/L benzylaminopurine, and 3 g/L Phytagel stimulated the formation of secondary embryos that resulted in development of large number of doubled haploid plants. [ABSTRACT FROM AUTHOR]