학술논문
Embryo viability indexing using Raman spectroscopy of spent culture media.
Document Type
Article
Author
Source
Subject
*EMBRYOS
*VIABILITY (Biology)
*CULTURE media (Biology)
*FERTILIZATION in vitro
*RAMAN spectroscopy
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Language
ISSN
0038-7010
Abstract
Embryo viability quantification is an important topic for in vitro fertilization researchers. This study suggests Raman spectroscopy as a candidate method to find an objective measure of embryo viability. Raman spectra of 31 spent embryo culture samples (16 nonpregnant, 15 pregnant) from 31 patients were analyzed with band component analysis. The band area ratio of 902 to 943 cm−1was found to be the most discriminatory. These bands are related to glutamine, glycine, proline, aspartic acid, and valine. According to the pregnancy outcome the sensitivity and specificity of Raman analysis were found to be 93% and 77%, respectively. [ABSTRACT FROM PUBLISHER]