학술논문

Delineating the Role of Toll-Like Receptors in Inflammatory Bowel Disease
Document Type
Chapter
Source
Toll-Like Receptors: Methods and Protocols. 08/22/2023. 2700:221-228
Subject
Toll-like receptor (TLR)
Inflammatory bowel disease (IBD)
Dextran sodium sulfate (DSS)
Colitis model
TLR knockout mouse
Language
English
ISSN
1064-3745
1940-6029
Abstract
Toll-like receptors (TLRs) Toll-like receptors (TLRs) recognize altered gut microbiota triggering an immune response. These responses play a critical role in the pathogenesis and treatment of inflammatory bowel disease (IBD). IBD is characterized by inflammation of the intestinal tracts as in Crohn’s disease and ulcerative colitis. However, one challenge in determining the role of a specific TLR Toll-like receptors (TLRs) in IBD and its underlying mechanism is disparity. Variance in age, gender, race, and ethnicity shows a dramatic difference in the disease incidence, severity, and response to treatment. Delineating the role of TLRs in IBD relies on both a knockout mouse and a disease model. Here, we describe a detailed protocol on how to use nearly identical genetic backgrounds of TLR Toll-like receptors (TLRs) wild-type and knockout littermate mice in a dextran sodium sulfate (DSS)-induced colitis model.