학술논문
HPLC法同时测定桑菊感冒颗粒中5种有效成分的含量 / Simultaneous Determination of 5 Effective Components in Sangju Ganmao Granules by HPLC
Document Type
Academic Journal
Source
中国药房 / China Pharmacy. 29(12):1609-1612
Subject
Language
Chinese
ISSN
1001-0408
Abstract
目的:建立同时测定桑菊感冒颗粒中绿原酸、芦丁、连翘苷、槲皮素和甘草酸含量的方法.方法:采用高效液相色谱法.色谱柱为WondasilTM-C18,流动相为乙腈-0.4%磷酸溶液(梯度洗脱),流速为0.8 mL/min,柱温为30℃,检测波长为254 nm,进样量为5 μL.结果:绿原酸、芦丁、连翘苷、槲皮素和甘草酸的检测进样量线性范围分别为0.014～0.560 μg(r＝0.999 2)、0.022～0.880 μg (r＝0.999 6)、0.010～0.400 μg(r＝0.999 9)、0.016～0.640 μg(r＝0.999 9)、0.011～0.440 μg(r＝0.999 1);定量限分别为0.752、0.647、0.483、0.372、0.242 mg/L,检测限分别为0.253、0.185、0.157、0.162、0.098 mg/L;精密度、稳定性、重复性试验的RSD均小于2.0%;加样回收率分别为97.4%～100.9%(RSD＝1.4%,n＝6)、95.7%～99.0%(RSD＝1.1%,n＝6)、95.1%～98.1%(RSD＝1.3%,n＝6)、96.4%～99.8%(RSD＝1.4%,n＝6)、97.8%～100.4%(RSD＝1.0%,n＝6).结论:该方法操作简便,精密度、稳定性、重复性好,可用于桑菊感冒颗粒中绿原酸、芦丁、连翘苷、槲皮素和甘草酸含量的同时测定.
OBJECTIVE:To establish a method for the content determination of chlorogenic acid,rutin,phillyrin,quercetin and glycyrrhizic acid in Sangju ganmao granules. METHODS:HPLC method was adopted. The determination was performed on WondasilTM-C18 column with mobile phase consisted of acetonitrile-0.4% phosphoric acid solution(gradient elution)at the flow rate of 0.8 mL/min. The column temperature was set at 30 ℃,and the detection wavelength was set at 254 nm. The sample size was 5 μL. RESULTS:The linear range of chlorogenic acid,rutin,phillyrin,quercetin and glycyrrhizic acid were 0.014-0.560 μg (r＝0.999 2),0.022-0.880 μg(r＝0.999 6),0.010-0.400 μg(r＝0.999 9),0.016-0.640 μg(r＝0.999 9),0.011-0.440 μg(r＝0.999 1), respectively. The limits of quantitation were 0.752,0.647,0.483,0.372,0.242 mg/L;the limits of detection were 0.253,0.185, 0.157,0.162,0.098 mg/L. RSDs of precision,stability and reproducibility tests were all lower than 2.0%. The average recoveries were 97.4%-100.9%(RSD＝1.4%,n＝6),95.7%-99.0%(RSD＝1.1%,n＝6),95.1%-98.1%(RSD＝1.3%,n＝6),96.4%-99.8%(RSD＝1.4%,n＝6),97.8%-100.4%(RSD＝1.0%,n＝6),respectively. CONCLUSIONS:The method is simple,precise,stable and reproducible,and can be used for simultaneous determination of chlorogenic acid,rutin,phillyrin,quercetin and glycyrrhizic acid in Sangju ganmao granules.
OBJECTIVE:To establish a method for the content determination of chlorogenic acid,rutin,phillyrin,quercetin and glycyrrhizic acid in Sangju ganmao granules. METHODS:HPLC method was adopted. The determination was performed on WondasilTM-C18 column with mobile phase consisted of acetonitrile-0.4% phosphoric acid solution(gradient elution)at the flow rate of 0.8 mL/min. The column temperature was set at 30 ℃,and the detection wavelength was set at 254 nm. The sample size was 5 μL. RESULTS:The linear range of chlorogenic acid,rutin,phillyrin,quercetin and glycyrrhizic acid were 0.014-0.560 μg (r＝0.999 2),0.022-0.880 μg(r＝0.999 6),0.010-0.400 μg(r＝0.999 9),0.016-0.640 μg(r＝0.999 9),0.011-0.440 μg(r＝0.999 1), respectively. The limits of quantitation were 0.752,0.647,0.483,0.372,0.242 mg/L;the limits of detection were 0.253,0.185, 0.157,0.162,0.098 mg/L. RSDs of precision,stability and reproducibility tests were all lower than 2.0%. The average recoveries were 97.4%-100.9%(RSD＝1.4%,n＝6),95.7%-99.0%(RSD＝1.1%,n＝6),95.1%-98.1%(RSD＝1.3%,n＝6),96.4%-99.8%(RSD＝1.4%,n＝6),97.8%-100.4%(RSD＝1.0%,n＝6),respectively. CONCLUSIONS:The method is simple,precise,stable and reproducible,and can be used for simultaneous determination of chlorogenic acid,rutin,phillyrin,quercetin and glycyrrhizic acid in Sangju ganmao granules.