부산대학교 도서관

Plant-derived smoke water (SW) and karrikinolide (KAR) have been advanced to promote the accumulation of active ingredients in traditional Chinese medicine. However, the understanding of which link the active ingredients and rhizosphere microorganisms is unclear. This research was carried out with the speculation that the reshaping of rhizosphere microorganisms is one of the possible mechanisms to promote the accumulation of active ingredients in Panax quinquefolium L. under SW and KAR treatments. This work aims to discuss the microbial features in the rhizosphere of P. quinquefolius under SW and KAR, and indirect effects on the active ingredients of P. quinquefolius. Therefore, P. quinquefolius was treated with SW and KAR irrigation, and a no added control (CK). SW and KAR treatments increased the active components content and rhizosphere soil enzymes activity of P. quinquefolius, and improved soil physicochemical properties that were more suitable for the growth of P. quinquefolius. This increase might be related to the changes in the structure and function of rhizosphere microbial communities. The relative abundance of beneficial soil microorganisms increased after treatment with SW and KAR. Glycosyl Transferases, the key enzymes in the ginsenosides biosynthesis, have been reprogrammed in soil microorganism. Moreover, there was a complex correlation between P. quinquefolius active ingredients and significantly different microorganisms, microbial function, such as Rb1, Re and Rg1 was significantly positively correlated with Deinococcus-Thermus, Candidatus Latescibacteria and GT26. Therefore, our result showed that the use of SW and KAR was conducive to improving the active ingredients of P. quinquefolius by affecting the structure of rhizosphere microbial community, which provides guidance for the application prospect of SW in the high-quality production of medicinal materials.