학술논문
l-Cysteine-Tuned the Hierarchical Structure Based on Benzimidazole: Synthesis, Characterization, and Application in Ratiometric Electrochemiluminescence Immunoassay.
Document Type
Academic Journal
Author
Dai YX; School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, PR China.; Li YX; School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, PR China.; Chauvin J; University of Grenoble Alpes-CNRS, DCM UMR 5250, F-38000 Grenoble, France.; Zhang XJ; School of Biomedical Engineering, Health Science Centre, Shenzhen University, Shenzhen 518060, PR China.; Cosnier S; University of Grenoble Alpes-CNRS, DCM UMR 5250, F-38000 Grenoble, France.; Centre for Organic and Nanohybrid Electronics, Silesian University of Technology, Konarskiego 22B, 44-100 Gliwice, Poland.; Department of Physical Chemistry and Technology of Polymers, Silesian University of Technology, M. Strzody 9, 44-100 Gliwice, Poland.; Marks RS; Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 90089, Israel.; Shan D; School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, PR China.
Source
Publisher: American Chemical Society Country of Publication: United States NLM ID: 101669031 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2379-3694 (Electronic) Linking ISSN: 23793694 NLM ISO Abbreviation: ACS Sens Subsets: MEDLINE
Subject
Language
English
Abstract
Efficient and robust electrochemiluminescence (ECL) emitters are crucial for enhancing the ECL immunosensor sensitivity. This study introduces a novel ECL emitter, CoBIM/Cys, featuring a hierarchical core-shell structure. The core of the structure is created through the swift coordination between the sulfhydryl and carboxyl groups of l-cysteine (l-Cys) and cobalt ions (Co 2+ ), while the shell is constructed by sequentially coordinating benzimidazole (BIM) with Co 2+ . This design yields a greater specific surface area and a more intricate porous structure compared to CoBIM, markedly enhancing mass transfer and luminophore accessibility. Moreover, the l-Cys and Co 2+ core introduces Co-S and Co-O catalytic sites, which improve the catalytic decomposition of H 2 O 2 , leading to an increased production of hydroperoxyl radicals (OOH • ). This mechanism substantially amplifies the ECL performance. Leveraging the competitive interaction between isoluminol and BIM for OOH • during ECL emission, we developed a ratiometric immunosensor for cardiac troponin I (cTnI) detection. This immunosensor demonstrates a remarkably broad detection range (1 pg mL -1 to 10 ng mL -1 ), a low detection limit (0.4 pg mL -1 ), and exceptional reproducibility and specificity.