부산대학교 도서관

Introduction: The COVID‑19 pandemic has resulted in an increased need for molecular diagnostics. Ribonucleic acid (RNA) extraction is a crucial step in the detection of severe acute respiratory syndrome coronavirus‑2 (SARS‑COV‑2). The resulting outcome depends on the performance of the RNA extraction kit used. Methods: This study evaluated the efficiency of four different commercial RNA extraction kits for the detection of SARS‑COV 2. The reproducibility and performance efficiency of each kit were assessed and compared. The performance, processing time and storage conditions were also considered in the evaluation. Results: A slightly lesser cycle threshold (Ct) values were observed in RNA extracted using HiPurA Viral RNA Purification Kit. Invitrogen PureLink RNA Mini Kit was found to be time‑saving and user‑friendly. Conclusions: All the RNA isolation kit yielded the best results; however, if time is the important considerations, Invitrogen PureLink RNA Mini Kit has the shortest processing time. [ABSTRACT FROM AUTHOR]