학술논문
Oxidative stress mediated cytogenotoxicological effects of phytol in wistar albino rats
Document Type
Original Paper
Author
de Alencar, Marcus Vinícius Oliveira Barros; Islam, Muhammad Torequl; dos Reis, Antonielly Campinho; de Oliveira Santos, José Victor; Nunes, Adriana Maria Viana; da Silva, Felipe Cavalcante Carneiro; da Conceição Machado, Keylla; de Castro e Sousa, João Marcelo; Reiner, Željko; Martorell, Miquel; Fagoonee, Sharmila; Sharifi-Rad, Javad; de Carvalho Melo-Cavalcante, Ana Amélia
Source
Advances in Traditional Medicine. 23(1):273-290
Subject
Language
English
ISSN
2662-4052
2662-4060
2662-4060
Abstract
Phytol (PHY), a diterpenoid, is known for its various bio-pharmacological activities. However, its toxicological profile has yet to be evaluated. The aim of this study was to evaluate the cytogenotoxicological profile of PHY in Wistar albino rats. Forty-five female non-pregnant rats were treated acutely and subchronically with PHY at doses of 300 and 2000 mg/kg and 30, 60 and 90 mg/kg for 14 and 28 days. Neuropharmacological, genotoxic, and mutagenic effects were investigated. The results suggest that PHY did not cause the death of rats at a dose of 2000 mg/kg, suggesting a higher range of the LD50 of this diterpenoid. Several toxicological alterations were observed in clinical and neuropharmacological parameters depending upon the doses. No hepatic histopathological changes were observed. PHY induced genotoxicity in peripheral blood, bone marrow, liver, and kidney. PHY did not show damage repair activity in peripheral blood lymphocytes. In the bone marrow, both acute and subchronic PHY treatments increased micronucleus frequency, indicating a mutagenic effect. PHY caused neuropharmacological alterations and genetic instability, possibly through the oxidative stress induction pathway.