부산대학교 도서관

The use of elicitors has become a valuable strategy to enhance the production of plant bioactive compounds. In this work jasmonic acid solutions and extracts containing jasmonates produced by Botryosphaeria rhodina were used to elicit coumarin accumulation in Mikania laevigata. Plants cultured inside a greenhouse under monitored environmental conditions were sprayed with jasmonic acid (JA) solutions (100 or 200 µM) or with B. rhodina fungal extracts (JE) containing equivalent concentrations of JA. After 1, 7 and 30 days of exposure elicited plants were extracted and HPLC/DAD analyzed to establish the chemical profile of constituents. Yields of coumarin in elicited plants were determined and it was observed that the coumarin production was influenced by JA concentration and plant exposure period, being significantly increased (16.98 and 24.76 mg/g DW) in plants elicited for 30 days with JE containing 100 and 200 µM of JA, respectively. Compared to control, the production of coumarin in elicited plants was 1.6 and 2.3 fold higher, respectively. Obtained results demonstrate that B. rhodina fungal extracts notably enhance coumarin production in M. laevigata. Overall results in this work corroborate the eliciting effect of jasmonates on the production of secondary metabolites in plants. Those findings support the development of an eco-friendly product containing JE, which could be applied to fertilize medicinal plant cultures and to enhance the production bioactives to be used as constituents in the formulation of innovative phytochemicals.