학술논문

Dengue virus type 3 isolation from Aedes aegypti in the municipality of Nova Iguaçu, State of Rio de Janeiro
Document Type
article
Source
Memórias do Instituto Oswaldo Cruz. September 2002 97(6)
Subject
dengue
Aedes aegypti
Aedes albopictus
reverse transcription
polymerase chain reaction
virus isolation
Rio de Janeiro
Brazil
Language
English
ISSN
0074-0276
Abstract
In a prospective field study conducted from July 2000 to June 2001, adult Aedes aegypti and Ae. albopictus mosquitoes were caught from the municipality of Nova Iguaçu, State of Rio de Janeiro, Brazil. Virus isolation in Ae. albopictus clone C6/36 cell line and a semi-nested reverse transcription-polymerase chain reaction detected only dengue virus type 3 in three pools of Ae. aegypti, despite the co-circulation of DEN-1, DEN-2 and DEN-3 serotypes in that area. No viruses were detected in Ae. albopictus mosquitoes. This virological surveillance consists in a sentinel system alerting for dengue outbreaks.