학술논문
Disulfide High‐Mobility Group Box 1 Drives Ischemia‐Reperfusion Injury in Human Liver Transplantation
Document Type
article
Author
Sosa, Rebecca A; Terry, Allyson Q; Kaldas, Fady M; Jin, Yi‐Ping; Rossetti, Maura; Ito, Takahiro; Li, Fang; Ahn, Richard S; Naini, Bita V; Groysberg, Victoria M; Zheng, Ying; Aziz, Antony; Nevarez‐Mejia, Jessica; Zarrinpar, Ali; Busuttil, Ronald W; Gjertson, David W; Kupiec‐Weglinski, Jerzy W; Reed, Elaine F
Source
Hepatology. 73(3)
Subject
Language
Abstract
Background and aimsSterile inflammation is a major clinical concern during ischemia-reperfusion injury (IRI) triggered by traumatic events, including stroke, myocardial infarction, and solid organ transplantation. Despite high-mobility group box 1 (HMGB1) clearly being involved in sterile inflammation, its role is controversial because of a paucity of patient-focused research.Approach and resultsHere, we examined the role of HMGB1 oxidation states in human IRI following liver transplantation. Portal blood immediately following allograft reperfusion (liver flush; LF) had increased total HMGB1, but only LF from patients with histopathological IRI had increased disulfide-HMGB1 and induced Toll-like receptor 4-dependent tumor necrosis factor alpha production by macrophages. Disulfide HMGB1 levels increased concomitantly with IRI severity. IRI+ prereperfusion biopsies contained macrophages with hyperacetylated, lysosomal disulfide-HMGB1 that increased postreperfusion at sites of injury, paralleling increased histone acetyltransferase general transcription factor IIIC subunit 4 and decreased histone deacetylase 5 expression. Purified disulfide-HMGB1 or IRI+ blood stimulated further production of disulfide-HMGB1 and increased proinflammatory molecule and cytokine expression in macrophages through a positive feedback loop.ConclusionsThese data identify disulfide-HMGB1 as a mechanistic biomarker of, and therapeutic target for, minimizing sterile inflammation during human liver IRI.