부산대학교 도서관

Atenolol and related compounds found in raw materials and commercial products were analyzed by reversed-phase high-performance liquid chromatography. A mixed solution of phosphate buffer (3.4 gil. pH 3.0). tetrahvdrofurane and methanol (800:20:180. v/v/v) including sodium octanesulfonate (1 g/l) and tetrabutylammoniumhvdrogensulfate (0.4 g/l) was used as mobile phase at the flow rate of 0.25 m1`min. Detection was carried out at UV 226 nm. Atenolol related compounds. such as bis ether. tertiary amine and blocker acid were identified by comparing the retention time of the standard. The within-day and between-day precisions of the separated compounds were less than 1.2"o and 3.4° o. respectively. The contents of related compounds of the tested samples were under the limit prescribed in the European Pharmacopoeia. The pattern of the related compounds showed that atenolol raw materials and products could be classified in three different groups. indicating that the materials originated from different source or treated in different way.