학술논문
Loss of EMP2 Inhibits Melanogenesis of MNT1 Melanoma Cells via Regulation of TRP-2
Document Type
Article
Author
Enkhtaivan Enkhmend; Kim Hyun Ji; Kim Boram; Byun Hyung Jung; Yu Lu; Nguyen Tuan Minh; Nguyen Thi Ha; Do Phuong Anh; Kim Eun Ji; Kim Kyung Sung; Huy Hiệu Phùng; Rahman Mostafizur; Jang Ji Yun; Rho Seung Bae; 이호; Kang Gyeoung Jin; Park Mi Kyung; Kim Nan-Hyung; Choi Chang Ick; Lee Kyeong; Han Hyo Kyung; Cho Jungsook; 이애영; Lee Chang Hoon
Source
Biomolecules & Therapeutics, 30(2), pp.203-211 Mar, 2022
Subject
Language
English
ISSN
2005-4483
1976-9148
1976-9148
Abstract
Melanogenesis is the production of melanin from tyrosine by a series of enzyme-catalyzed reactions, in which tyrosinase and DOPA oxidase play key roles. The melanin content in the skin determines skin pigmentation. Abnormalities in skin pigmentation lead to various skin pigmentation disorders. Recent research has shown that the expression of EMP2 is much lower in melanoma than in normal melanocytes, but its role in melanogenesis has not yet been elucidated. Therefore, we investigated the role of EMP2 in the melanogenesis of MNT1 human melanoma cells. We examined TRP-1, TRP-2, and TYR expression levels during melanogenesis in MNT1 melanoma cells by gene silencing of EMP2. Western blot and RT-PCR results confirmed that the expression levels of TYR and TRP-2 were decreased when EMP2 expression was knocked down by EMP2 siRNA in MNT1 cells, and these changes were reversed when EMP2 was overexpressed. We verified the EMP2 gene was knocked out of the cell line (EMP2 CRISPR/Cas9) by using a CRISPR/Cas9 system and found that the expression levels of TRP-2 and TYR were significantly lower in the EMP2 CRISPR/Cas9 cell lines. Loss of EMP2 also reduced migration and invasion of MNT1 melanoma cells. In addition, the melanosome transfer from the melanocytes to keratinocytes in the EMP2 KO cells cocultured with keratinocytes was reduced compared to the cells in the control coculture group. In conclusion, these results suggest that EMP2 is involved in melanogenesis via the regulation of TRP-2 expression.