학술논문
High-level expression and characterization of Aspergillus niger ATCC 1015 xylanase B in Komagataella phaffii
Document Type
Article
Author
Source
Applied Biological Chemistry, 61(4), pp.373-381 Aug, 2018
Subject
Language
English
ISSN
2468-0842
2468-0834
2468-0834
Abstract
Owing to the safety issues in food and feed industry, the GH11 xylanase B gene from Aspergillus niger ATCC 1015 was cloned and expressed in Komagataella phaffii. The highest xylanase B activity of 1827.19 U/ml was obtained after optimization of temperature, pH, and methanol addition through flask cultivation. The optimal temperature and pH were 55 C and 5.0, respectively, and the highest relative activity of xylanase B reached 133.20% with the addition of 10 mmol/l cupric ions. Thus, the highlevel recombinant xylanase B obtained in this study could have potential applications in food and feed industry.