부산대학교 도서관

To authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1600-0625.2008.00787.x Byline: Gaelle Quereux (1), Elisabeth Andre-Garnier (2), Anne-Chantal Knol (1), Berthe-Marie Imbert-Marcille (2), Brigitte Dreno (1) Keywords: human herpes virus-6; human herpes virus-8; mycosis fungoides; parapsoriasis Abstract: Abstract Introduction: The aetiology of mycosis fungoides and parapsoriasis (which may be considered as an early stage of mycosis fungoides) remains debated. Previous recent studies have suspected the involvement of viral agents and particularly human herpes viruses (HHV).The aim of the present study was to screen for the presence of HHV-6 and HHV-8 genome in parapsoriasis samples. Method: Fifty paraffin-embedded samples from skin biopsies of parapsoriasis were retrospectively collected from archival files in our Dermatology department. Total DNA was extracted from samples using the phenol-chloroform method and the presence of viral genomes was screened using real-time PCR. Results: Forty nine out of the fifty tissue samples of parapsoriasis were interpretable, they were all found negative for HHV-6 and HHV-8. Discussion: This study does not confirm the suspected role of HHV-6 or -8 in parapsoriasis. HHV-8 has been the most studied virus in parapsoriasis and more widely in cutaneous lymphoproliferative diseases and our results are in agreement with most of the studies which found none or few HHV-8 in more advanced stages of cutaneous lymphoproliferative diseases. Concerning HHV-6, our study is the first one investigating the presence of this virus in lesional tissue samples of patients with parapsoriasis. In conclusion, parapsoriasis does not seem to be associated with either HHV-6 or HHV-8. Author Affiliation: (1)Skin Cancer Unit (2)EA 4271 Virology Laboratory, Nantes University Hospital, Nantes, France Article History: Accepted for publication 8 July 2008 Article note: Prof. Dreno Brigitte, MD, PHD, INSERM 892, Unit of Skin Cancer, Nantes University Hospital, 1 Pl. A. Ricordeau, 44093 Nantes Cedex 1, France, Tel.: 00 240 083 118, Fax: 00 240 083 117, e-mail: bdreno@wanadoo.fr