학술논문
Calyptranthes grandifolia O.Berg (Myrtaceae) ethanolic extract inhibits TNF-[alpha] gene expression and cytokine release in vitro
tumor necrosis factor
tumor necrosis factor
Document Type
Report
Author
Source
Molecular Medicine Reports. May 2017, Vol. 15 Issue 5, p2873, 8 p.
Subject
Language
English
ISSN
1791-2997
Abstract
Introduction Inflammation associated with cancer is a promising target for the development of anticancer therapies. Cytokines, chemokines and growth factors may have an important function in the interaction between tumor [...]
Anti-tumor therapies based on anti-inflammatory effects have been considered in cancer treatment. Survival, proliferation and, resultantly, invasion and metastasis of tumor cells are regulated by local inflammatory mediators. Primary inflammatory cytokines, such as tumor necrosis factor (TNF), are targets for anticancer therapy. Several anti-inflammatory agents isolated from natural products are becoming important chemopreventive and therapeutic agents for cancer. The present study aimed to investigate the expression of TNF-[alpha], nuclear factor-[kappa]B (NF-[kappa]B) and p38[alpha] mitogen-activated protein kinase (p38[alpha]) genes, associated with proliferation and inflammation in the Caco-2 cell line treated with ethanolic and hexanic extracts of Calyptranthes grandifolia O.Berg (Myrtaceae). Caco-2 cells were cultured and treated with plant extract at different concentrations (25, 50, 100 and 200 [micro]g/ml) and stimulated with lipopolysaccharide (LPS). For gene expression, analysis was performed by total RNA extraction followed by synthesis of complementary DNA and analysis by quantitative polymerase chain reaction. The release of TNF-[alpha] cytokine was evaluated by ELISA in RAW 264.7 murine macrophages activated by LPS. Among the evaluated genes, there was a decrease in TNF-[alpha] expression at 100 and 200 [micro]g/ml concentrations only with the ethanolic extract (P Key words: inflammation, cancer, gene expression, cytokine, Calyptranthes grandifolia
Anti-tumor therapies based on anti-inflammatory effects have been considered in cancer treatment. Survival, proliferation and, resultantly, invasion and metastasis of tumor cells are regulated by local inflammatory mediators. Primary inflammatory cytokines, such as tumor necrosis factor (TNF), are targets for anticancer therapy. Several anti-inflammatory agents isolated from natural products are becoming important chemopreventive and therapeutic agents for cancer. The present study aimed to investigate the expression of TNF-[alpha], nuclear factor-[kappa]B (NF-[kappa]B) and p38[alpha] mitogen-activated protein kinase (p38[alpha]) genes, associated with proliferation and inflammation in the Caco-2 cell line treated with ethanolic and hexanic extracts of Calyptranthes grandifolia O.Berg (Myrtaceae). Caco-2 cells were cultured and treated with plant extract at different concentrations (25, 50, 100 and 200 [micro]g/ml) and stimulated with lipopolysaccharide (LPS). For gene expression, analysis was performed by total RNA extraction followed by synthesis of complementary DNA and analysis by quantitative polymerase chain reaction. The release of TNF-[alpha] cytokine was evaluated by ELISA in RAW 264.7 murine macrophages activated by LPS. Among the evaluated genes, there was a decrease in TNF-[alpha] expression at 100 and 200 [micro]g/ml concentrations only with the ethanolic extract (P Key words: inflammation, cancer, gene expression, cytokine, Calyptranthes grandifolia