부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.jphotobiol.2009.06.002 Byline: Na Li (a), Han-Hong Xu (a), Zheng-Yong Liu (b), Zhuo-Hong Yang (b) Keywords: Pyrethroid insecticide; [alpha]-terthienyl; Photoactivated cytotoxicity; Spodoptera litura cell; ROS; MMP Abstract: The synthesis of one pyrethroid insecticide [2-methyl-4-oxo-3-prop-2-yn-1-ylcyclopent-2-en-1-yl-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate (Abbrev. JZ) (Fig. 1)] conjugated with a series of [alpha]-terthienyl derivatives (2-8) (Fig. 1) by palladium/copper-catalyzed cross-coupling reaction is presented here for evaluating the photoactivated cytotoxicity. The photoactivated cytotoxicity on Spodoptera litura (SL) cell line was detected by MTT assay. The inhibitory activity of all the conjugates was enhanced in the irradiation condition, compared with that of JZ. The IC.sub.50 values of the most effective compound 9 (Fig. 1) treated with irradiation were 11.60[mu]gmL.sup.-1 at 24h and 8.93[mu]gmL.sup.-1 at 48h, respectively. Generation of intracellular reactive oxygen species (ROS) and change of mitochondrial transmembrane potential (MMP) on SL cells treated with compound 9 were used for the further photoactivated study. A summary of these experiments on compound 9 demonstrated the notable ROS generation and dramatic MMP decrease when irradiated with UVA light. The results also represented statistically significant difference between dark and irradiation treatment of compound 9. However, in control and JZ groups, the effects were not statistically different. It was proved that our prepared compounds were ideal candidates for new photoactivated pyrethroid insecticides. Author Affiliation: (a) Laboratory of Insect Toxicology and Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, China (b) College of Science, South China Agricultural University, Guangzhou, Guangdong 510642, China Article History: Received 16 February 2009; Revised 2 June 2009; Accepted 9 June 2009