학술논문
FSH3 mediated cell death is dependent on NUC1 in Saccharomyces cerevisiae
RESEARCH ARTICLE
RESEARCH ARTICLE
Document Type
Report
Source
FEMS Yeast Research. May 2019, Vol. 19 Issue 3, p1g, 14 p.
Subject
Language
English
ISSN
1567-1356
Abstract
INTRODUCTION Cell development and homeostasis in multicellular organisms is related with the removal of unwanted or damaged cells, response to biotic and abiotic stresses, differentiation and aging (Green 2005;Buttner et [...]
Family of Serine Hydrolases (FSH) members FSH1, FSH2 and FSH3 in Saccharomyces cerevisiae share conserved sequences with the human candidate tumor suppressor OVCA2. In this study, hydrogen peroxide ([H.sub.2][O.sub.2]) exposure increased the expression of both mRNA and protein levels of FSH3 in wild-type (WT) yeast cells. The deletion of FSH3 improved the yeast growth rate under [H.sub.2][O.sub.2]-induction as compared to WT control cells. The overexpression of FSH3 in WT yeast cells caused an apoptotic phenotype, including accumulation of reaction oxygen species, decreased cell viability and cell death. The double deletions fsh1[DELTA] fsh2[DELTA], fsh1[DELTA] fsh3[DELTA] and fsh2[DELTA] fsh3[DELTA] displayed increased growth compared to WT cells. However, the overexpression of FSH3 effectively inhibited cell growth in all double deletions. Moreover, the overexpression of FSH3 in cells lacking NUC1 did not cause any growth defect in the presence or absence of [H.sub.2][O.sub.2] Our results suggest that FSH3 induced apoptosis of yeast in a NUC1 dependent manner. Keywords: family of serine hydrolases; apoptosis; FSH3; chronological life span; hydrogen peroxide; NUC1
Family of Serine Hydrolases (FSH) members FSH1, FSH2 and FSH3 in Saccharomyces cerevisiae share conserved sequences with the human candidate tumor suppressor OVCA2. In this study, hydrogen peroxide ([H.sub.2][O.sub.2]) exposure increased the expression of both mRNA and protein levels of FSH3 in wild-type (WT) yeast cells. The deletion of FSH3 improved the yeast growth rate under [H.sub.2][O.sub.2]-induction as compared to WT control cells. The overexpression of FSH3 in WT yeast cells caused an apoptotic phenotype, including accumulation of reaction oxygen species, decreased cell viability and cell death. The double deletions fsh1[DELTA] fsh2[DELTA], fsh1[DELTA] fsh3[DELTA] and fsh2[DELTA] fsh3[DELTA] displayed increased growth compared to WT cells. However, the overexpression of FSH3 effectively inhibited cell growth in all double deletions. Moreover, the overexpression of FSH3 in cells lacking NUC1 did not cause any growth defect in the presence or absence of [H.sub.2][O.sub.2] Our results suggest that FSH3 induced apoptosis of yeast in a NUC1 dependent manner. Keywords: family of serine hydrolases; apoptosis; FSH3; chronological life span; hydrogen peroxide; NUC1