학술논문
Regulation of magnesium-inhibited cation current by actin cytoskeleton rearrangement
Document Type
Report
Author
Source
Biochemical and Biophysical Research Communications. Jan 20, 2006, Vol. 339 Issue 3, p810, 6 p.
Subject
Language
English
ISSN
0006-291X
Abstract
To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.bbrc.2005.11.093 Byline: Soon Youn Jeong (a), Sun Young Shin (a), Han-Seop Kim (b), Chang-Dae Bae (b), Dae-Yong Uhm (a), Myung-Kyu Park (a), Sungkwon Chung (a) Keywords: MIC; CRAC; TRPM7; Actin cytoskeleton; RhoA Abstract: Magnesium-inhibited, non-selective cation current (I.sub.MIC) is activated by depletion of intracellular Mg.sup.2+ and ATP. I.sub.MIC transports various divalent cations including Mg.sup.2+ and Ca.sup.2+, and is involved in cell viability. We investigated the effect of actin dynamics on I.sub.MIC. Formation of a stable cortical actin network by calyculin A inhibited the activation of I.sub.MIC, while the actin depolymerizing reagent, cytochalasin D, reversed the inhibition. Induction of a dense cortical actin layer by transfecting the constitutively active form of RhoA also inhibited the activation of I.sub.MIC. These results suggest that the activation of I.sub.MIC may be dynamically regulated by actin cytoskeleton rearrangement. Author Affiliation: (a) Department of Physiology, Sungkyunkwan University School of Medicine, Suwon 440-746, South Korea (b) Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon 440-746, South Korea Article History: Received 9 November 2005