학술논문
Scavenging Activity on Reactive Oxygen Species with Biological Relevance by Varronia curassavica
Document Type
Academic Journal
Author
Source
Orbital: The Electronic Journal of Chemistry. April-June, 2023, Vol. 15 Issue 2, p111, 7 p.
Subject
Language
English
ISSN
1984-6428
Abstract
1. Introduction Varronia curassavica Jacq. (Boraginaceae), a shrub known as erva-baleeira, is a medicinal plant and a non-conventional food plant found in Brazil, mainly in the Atlantic Forest and South [...]
Varronia curassavica Jacq. is a medicinal plant found in Brazil used as anti-inflammatory. Here, we investigated the in vitro antioxidant activity of 70 % ethanol extract of V curassavica leaves on synthetic radicals (ABTS*[+ or -]/DPPH*) and reactive oxygen species ([O.sub.2.sup.*-], ROO*, HOCl/OCl-, [H.sub.2][O.sub.2]), besides its in vitro cytotoxicity. The extract was characterized by UPLC-ESI-QToF-[MS.sup.E] and the annotated compounds were one hydroxybenzoic acid, five phenylpropanoids, and three glycosylated quercetin derivatives, being the main compound rosmarinic acid or its isomer. The antioxidant activity was very promising in all tests, highlighting on the capture of [O.sup.2*-] which [EC.sub.50] value was three times lower than Trolox. This activity may be due to the presence of the major compounds, all phenolic compounds. The extract also presented low cytotoxicity. Thus, the extract from V curassavica leaves has great potential as an antioxidant. Keywords Antioxidant Reactive oxygen species (ROS) Oxidative stress Cytotoxicity
Varronia curassavica Jacq. is a medicinal plant found in Brazil used as anti-inflammatory. Here, we investigated the in vitro antioxidant activity of 70 % ethanol extract of V curassavica leaves on synthetic radicals (ABTS*[+ or -]/DPPH*) and reactive oxygen species ([O.sub.2.sup.*-], ROO*, HOCl/OCl-, [H.sub.2][O.sub.2]), besides its in vitro cytotoxicity. The extract was characterized by UPLC-ESI-QToF-[MS.sup.E] and the annotated compounds were one hydroxybenzoic acid, five phenylpropanoids, and three glycosylated quercetin derivatives, being the main compound rosmarinic acid or its isomer. The antioxidant activity was very promising in all tests, highlighting on the capture of [O.sup.2*-] which [EC.sub.50] value was three times lower than Trolox. This activity may be due to the presence of the major compounds, all phenolic compounds. The extract also presented low cytotoxicity. Thus, the extract from V curassavica leaves has great potential as an antioxidant. Keywords Antioxidant Reactive oxygen species (ROS) Oxidative stress Cytotoxicity