학술논문
Evaluation of Derma Genie™-H001 as a Functional Cosmetic Ingredient for Hair Loss Improvement: Assessing Anti-inflammatory, Antioxidative, Photoprotective, Antiandrogenic, and Hair Growth-Inductive Effects
탈모 증상 완화 및 육모 화장품 소재로써 Derma GenieTM-H001의 생리활성 효능 평가
탈모 증상 완화 및 육모 화장품 소재로써 Derma GenieTM-H001의 생리활성 효능 평가
Document Type
Article
Text
Text
Author
Seokmuk Park; Nayeon Han; Bo Liu; Younsang Choi; Seunghee Bae; Sungkwan An; Jungmin Lee; 박석묵; 한나연; 유보; 최윤상; 배승희; 안성관; 이정민
Source
아시안뷰티화장품학술지, 03/30/2024, Vol. 22, Issue 1, p. 75-89
Subject
Language
Korean
ISSN
2466-2046
Abstract
目的: 本通过评估 Derma GenieTM-H001 对抗炎、抗氧化、光保护、抗雄激素和激活毛发生长相关基因表达的 影响，评估 Derma GenieTM-H001作为缓解脱发症状的功能性化妆品成分的潜力。方法: 我们采用了多种方法， 包括一氧化氮(NO)测定和qRT-PCR，以评估抗炎活性。此外，本研究使用WST-1测定、DCF-DA 测定、LDH leakage assay, UVB-irradiation assay 评估了对人真皮乳头细胞 (HDPC) 的抗氧化和光保护作用。为了研究 Derma GenieTM-H001 的抗脱发效果，进行了3D球体培养和qRT-PCR。结果: Derma GenieTM-H001表现出多酚 的特性，并表现出 DPPH 自由基清除活性。重要的是，它对HDPC没有细胞毒性，并且增加了HDPC 3D 球体的 直径。在用Derma GenieTM处理的Raw 264.7细胞中，NO和促炎细胞因子等炎症介质以剂量依赖性方式减少。 此外，Derma GenieTM对HDPC中的过氧化氢(H2O2)和UVB诱导的细胞毒性表现出显着的保护作用。值得注意的 是，qRT-PCR 分析显示，在经Derma GenieTM处理的HDPC中，与脱发相关的基因表达减少，与毛发生长相关 的基因表达增加。结论: Derma Genie™-H001在减轻LPS诱导的促炎细胞因子、H2O2诱导的细胞毒性、UVB 诱 导的细胞毒性和DHT诱导的TGF-β1表达方面表现出良好的效果。此外，它还显着上调头发生长相关基因的表 达。这些发现表明Derma Genie™可作为一种有价值的功能性化妆品成分，用于改善脱发症状。
Purpose: This study aimed to assess the potential of Derma Genie™-H001 as a functional cosmetic ingredient for alleviating hair loss symptoms by evaluating its impact on anti-inflammatory, antioxidative, photoprotective, antiandrogenicity, and activation of hair growth-related gene expression. Methods: We employed various methods, including nitric oxide (NO) assay and quantitative real-time polymerase chain reaction (qRT-PCR, to estimate anti-inflammatory activities). Additionally, this study assessed antioxidative and photoprotective effects on human dermal papilla cells (HDPCs) using water-soluble tetrazolium salt (WST-1) assay, 2′,7′-dichlorofluorescin diacetate (DCF-DA) assay, lactate dehydrogenase (LDH) assay, and ultraviolet B (UVB)-irradiation assay. To investigate the anti-hair loss effects of Derma Genie™-H001, 3D spheroid culture and qRT-PCR were conducted. Results: Derma Genie™-H001 exhibited the characteristics of polyphenols and demonstrated 2,2-dipheny-1-picrylhydrazyl (DPPH) radical scavenging activity. Importantly, it showed no cytotoxicity on HDPCs and increased the diameter of HDPCs 3D spheroid. Inflammatory mediators such as NO and proinflammatory cytokines were reduced in a dose-dependent manner in Raw 264.7 cells treated with Derma Genie™. Moreover, Derma Genie™ exhibited substantial protective effects against hydrogen peroxide (H2O2) and UVB-induced cytotoxicity in HDPCs. Notably, qRT-PCR analysis revealed a decrease in the expression of genes related to hair loss and an increase in the expression of genes associated with hair growth in HDPCs treated with Derma Genie™. Conclusion: Derma Genie™-H001 demonstrated promising effects in mitigating LPS-induced proinflammatory cytokines, H2O2-induced cytotoxicity, UVB-induced cytotoxicity, and DHT-induced TGF-β1 expression. Furthermore, it significantly upregulated the expression of hair growth-related genes. These findings suggest that Derma Genie™ may serve as a valuable functional cosmetic ingredient for improving hair loss symptoms.
Purpose: This study aimed to assess the potential of Derma Genie™-H001 as a functional cosmetic ingredient for alleviating hair loss symptoms by evaluating its impact on anti-inflammatory, antioxidative, photoprotective, antiandrogenicity, and activation of hair growth-related gene expression. Methods: We employed various methods, including nitric oxide (NO) assay and quantitative real-time polymerase chain reaction (qRT-PCR, to estimate anti-inflammatory activities). Additionally, this study assessed antioxidative and photoprotective effects on human dermal papilla cells (HDPCs) using water-soluble tetrazolium salt (WST-1) assay, 2′,7′-dichlorofluorescin diacetate (DCF-DA) assay, lactate dehydrogenase (LDH) assay, and ultraviolet B (UVB)-irradiation assay. To investigate the anti-hair loss effects of Derma Genie™-H001, 3D spheroid culture and qRT-PCR were conducted. Results: Derma Genie™-H001 exhibited the characteristics of polyphenols and demonstrated 2,2-dipheny-1-picrylhydrazyl (DPPH) radical scavenging activity. Importantly, it showed no cytotoxicity on HDPCs and increased the diameter of HDPCs 3D spheroid. Inflammatory mediators such as NO and proinflammatory cytokines were reduced in a dose-dependent manner in Raw 264.7 cells treated with Derma Genie™. Moreover, Derma Genie™ exhibited substantial protective effects against hydrogen peroxide (H2O2) and UVB-induced cytotoxicity in HDPCs. Notably, qRT-PCR analysis revealed a decrease in the expression of genes related to hair loss and an increase in the expression of genes associated with hair growth in HDPCs treated with Derma Genie™. Conclusion: Derma Genie™-H001 demonstrated promising effects in mitigating LPS-induced proinflammatory cytokines, H2O2-induced cytotoxicity, UVB-induced cytotoxicity, and DHT-induced TGF-β1 expression. Furthermore, it significantly upregulated the expression of hair growth-related genes. These findings suggest that Derma Genie™ may serve as a valuable functional cosmetic ingredient for improving hair loss symptoms.