학술논문
An ultrasonic method for filtering and separating cell suspensions
Document Type
Conference
Author
Source
1995 IEEE Ultrasonics Symposium. Proceedings. An International Symposium Ultrasonics Ultrasonics Symposium, 1995. Proceedings., 1995 IEEE. 2:1069-1072 vol.2 1995
Subject
Language
ISSN
1051-0117
Abstract
Continuous flow filtration of cell suspensions has recently become possible using ultrasound standing wave enhanced sedimentation. A filter which can be operated at either 1 or 3 MHz is described. At both 1 MHz and 3 MHz yeast cells (4.6 /spl mu/m diameter) can be filtered at greater than 99% efficiency at a flow rate of 5 ml min/sup -1/. Smaller cells such as E. coli (1.3 /spl mu/m diameter) were efficiently filtered at 3 MHz. This frequency dependent filtration suggests two modes of operations as (1) a filter to remove cells from suspension, or (2) a separator to separate a mixture of large (yeast) and small (E. coli) cells.