학술논문
Analysis of protein-DNA interactions in chromatin by UV induced cross-linking and mass spectrometry
Document Type
article
Author
Source
Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
Subject
Language
English
ISSN
2041-1723
Abstract
Cross-linking mass spectrometry (XLMS) allows mapping of protein-protein and protein-RNA interactions, but the analysis of protein-DNA complexes remains challenging. Here, the authors develop a UV light-based XLMS workflow to determine protein-DNA interfaces in reconstituted chromatin and isolated nuclei.