학술논문
Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen
Document Type
article
Author
Alice Mac Kain; Ghizlane Maarifi; Sophie-Marie Aicher; Nathalie Arhel; Artem Baidaliuk; Sandie Munier; Flora Donati; Thomas Vallet; Quang Dinh Tran; Alexandra Hardy; Maxime Chazal; Françoise Porrot; Molly OhAinle; Jared Carlson-Stevermer; Jennifer Oki; Kevin Holden; Gert Zimmer; Etienne Simon-Lorière; Timothée Bruel; Olivier Schwartz; Sylvie van der Werf; Nolwenn Jouvenet; Sébastien Nisole; Marco Vignuzzi; Ferdinand Roesch
Source
Nature Communications, Vol 13, Iss 1, Pp 1-13 (2022)
Subject
Language
English
ISSN
2041-1723
Abstract
Here, Mac Kain and Maarifi et al. perform a functional CRISPR/Cas9 screen to identify SARS-CoV-2 restriction factors in A549 cells. They identify DAXX, a scaffold protein of nuclear bodies with diverse functions, that has anti-viral activity post SARS-CoV-2 entry, while SARS-CoV-2 has evolved a mechanism to counteract its action via PLpro-mediated proteasomal degradation.