학술논문
Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice
Document Type
article
Author
Roman Maresch; Sebastian Mueller; Christian Veltkamp; Rupert Öllinger; Mathias Friedrich; Irina Heid; Katja Steiger; Julia Weber; Thomas Engleitner; Maxim Barenboim; Sabine Klein; Sandra Louzada; Ruby Banerjee; Alexander Strong; Teresa Stauber; Nina Gross; Ulf Geumann; Sebastian Lange; Marc Ringelhan; Ignacio Varela; Kristian Unger; Fengtang Yang; Roland M. Schmid; George S. Vassiliou; Rickmer Braren; Günter Schneider; Mathias Heikenwalder; Allan Bradley; Dieter Saur; Roland Rad
Source
Nature Communications, Vol 7, Iss 1, Pp 1-13 (2016)
Subject
Language
English
ISSN
2041-1723
Abstract
CRISPR/Cas9 technology has been used for genome engineering in vivo. Here, the authors use a transfection technique to deliver multiple guide RNAs to the pancreas of adult mice, allowing genetic screening and chromosome engineering in pancreatic cancer.