학술논문
Rapid and Flexible Platform To Assess Anti-SARS-CoV-2 Antibody Neutralization and Spike Protein-Specific Antivirals
Document Type
article
Author
Debora Stelitano; Stuart P. Weisberg; Monica P. Goldklang; Yun Zhu; Francesca T. Bovier; Gavreel F. Kalantarov; Giuseppe Greco; Didier Decimo; Gianluigi Franci; Michele Cennamo; Giuseppe Portella; Massimiliano Galdiero; Cyrille Mathieu; Branka Horvat; Ilya N. Trakht; Anne Moscona; Michael A. Whitt; Matteo Porotto
Source
mSphere, Vol 6, Iss 4 (2021)
Subject
Language
English
ISSN
2379-5042
Abstract
Assays for rapid biosafety level 2 (BSL2) evaluation of neutralizing properties of antibodies acquired via natural infection or through vaccination is urgently needed. Here, we propose a combinatorial approach in which sera are screened for SARS-CoV-2 spike protein (S) binding using a cell-based immunofluorescent (CBI) assay, and positive samples are further evaluated in a pseudotyped viral multicycle infection-mimicking protocol under BSL2 conditions.