학술논문
Thermostable group II intron reverse transcriptase fusion proteins and their use in cDNA synthesis and next-generation RNA sequencing
Document Type
Article
Author
Source
RNA; July 2013, Vol. 19 Issue: 7 p958-970, 13p
Subject
Language
ISSN
13558382; 14699001
Abstract
This paper describes unusual properties of group II intron-encoded reverse transcriptases. They have higher processivity, fidelity, and thermostability than retroviral enzymes. Moreover, they have an unexpected proclivity for template switching that makes them very useful for a number of cloning applications.