학술논문
Pro-inflammatory gene expression profile in obese adults with high plasma GIP levels
Document Type
Article
Author
Source
International Journal of Obesity (formerly International Journal of Obesity and Related Metabolic Disorders); April 2018, Vol. 42 Issue: 4 p826-834, 9p
Subject
Language
ISSN
03070565; 14765497
Abstract
Background:Glucose-dependent insulinotropic peptide (GIP) provides a novel link between the immune system and the gut, although results from different experimental and observational studies are contradictory, ranging from anti-inflammatory, through neutral to pro-inflammatory action of GIP. Thus, the aim of this study was to analyze inflammatory pathways on the level of gene expression and circulating inflammatory markers in relation to plasma GIP level.Subjects/Methods:The study included 128 obese adults. Two groups of obese subjects were created according to fasting GIP levels, with cutoff point at the 66th percentile and compared in respect with molecular and circulating markers of inflammation. GIP, interleukin (IL)-6 and adipokines: leptin, adiponectin, visfatin were measured by enzyme-linked immunosorbent assay. Inflammatory markers: monocyte chemoattractant protein-1 (MCP-1), sE-Selectin, sVCAM-1, sPECAM-1 were studied at fasting and after nutrient challenges. Gene expression in blood cells was determined by human gene microarray.Results:Obese patients with high GIP levels had elevated fasting glucose (Q2 (Q1–Q3): 5.6 (5.0–6.0) vs 5.0 (4.8–5.4), P<0.001), homeostasis model assessment of insulin resistance (Q2 (Q1–Q3): 3.68 (2.72–5.42) vs 2.70 (2.13–4.33), P=0.021), thus increased markers of insulin resistance as well as elevated inflammatory markers Il-6 (Q2 (Q1–Q3): 1.34 (1.0–2.04) vs 1.12 (0.76–1.64), P=0.045), MCP-1 (Q2 (Q1–Q3): 363 (287–447) vs 323 (263–389), P=0.026). Leptin to adiponectin ratio was significantly associated with fasting plasma GIP levels (β (95% CI): 0.84 (0.10–1.59)) independently of glucose levels. sE-Selectin was found to be a factor influencing GIP response to oral glucose intake (β (95% CI): 0.47 (0.14–0.81)) and sVCAM was found to be a factor influencing GIP response to high-fat meal intake (β (95% CI): 0.19 (0.01–0.37)). We identified 32 genes of inflammatory pathways differentially expressed in subjects with a high plasma GIP level compared to low GIP. Most upregulated genes play a role in leukocyte chemotaxis and tissue infiltration.Conclusions:These findings support the hypothesis that increased GIP signaling has a role in chronic low-grade inflammation.