학술논문
In vitroinduction of trained immunity in adherent human monocytes
Document Type
Article
Author
Domínguez-Andrés, Jorge; Arts, Rob J.W.; Bekkering, Siroon; Bahrar, Harsh; Blok, Bastiaan A.; de Bree, L. Charlotte J.; Bruno, Mariolina; Bulut, Özlem; Debisarun, Priya A.; Dijkstra, Helga; Cristina dos Santos, Jéssica; Ferreira, Anaísa V.; Flores-Gomez, Daniela; Groh, Laszlo A.; Grondman, Inge; Helder, Leonie; Jacobs, Cor; Jacobs, Liesbeth; Jansen, Trees; Kilic, Gizem; Klück, Viola; Koeken, Valerie A.C.M.; Lemmers, Heidi; Moorlag, Simone J.C.F.M.; Mourits, Vera P.; van Puffelen, Jelmer H.; Rabold, Katrin; Röring, Rutger J.; Rosati, Diletta; Tercan, Helin; van Tuijl, Julia; Quintin, Jessica; van Crevel, Reinout; Riksen, Niels P.; Joosten, Leo A.B.; Netea, Mihai G.
Source
STAR Protocols; 20210101, Issue: Preprints
Subject
Language
ISSN
26661667
Abstract
A growing number of studies show that innate immune cells can undergo functional reprogramming, facilitating a faster and enhanced response to heterologous secondary stimuli. This concept has been termed “trained immunity.” We outline here a protocol to recapitulate this in vitrousing adherent monocytes from consecutive isolation of peripheral blood mononuclear cells. The induction of trained immunity and the associated functional reprogramming of monocytes is described in detail using β-glucan (from Candida albicans) and Bacillus Calmette-Guérin as examples.