학술논문
Detection of IgG antibody to measles virus by radioimmunoassay technique.
Document Type
Academic Journal
Author
Source
Publisher: National Institute of Health, National Institute of Infectious Diseases Country of Publication: Japan NLM ID: 0243706 Publication Model: Print Cited Medium: Print ISSN: 0021-5112 (Print) Linking ISSN: 00215112 NLM ISO Abbreviation: Jpn J Med Sci Biol Subsets: MEDLINE
Subject
Language
English
ISSN
0021-5112
Abstract
A highly sensitive procedure of solid-phase radioimmunoassay (RIA) was developed for the detection of measles IgG antibody. HeLa cells persistently infected with measles virus were used as a solid-phase antigen. This technique was applied to the detection of measles IgG antibody in patients with subacute sclerosing panencephalitis (SSPE) and multiple sclerosis. Normal subjects having experienced natural measles or measles vaccination and patients with various neurological diseases of non-virus nature were also examined as control groups. Measles antibody was detected at high titers in both the sera and cerebrospinal fluid of SSPE patients. Moreover, RIA/HI ratios of SSPE patients were significantly higher than those of normal subjects, suggesting the presence in the formers of antibodies to nucleocapsids at high titers as well as to viral envelopes. On the other hand, no significant difference was found in both RIA and HI titers between the sera of multiple sclerosis and those of various neurological diseases.