학술논문
Imaging tumor microscopic viscosity in vivo using molecular rotors.
Document Type
Academic Journal
Author
Shimolina LE; Institute of Biomedical Technologies, Nizhny Novgorod State Medical Academy, Minin and Pozharsky Square, 10/1, Nizhny Novgorod, 603005, Russia.; Institute of Biology and Biomedicine, Nizhny Novgorod State University, Gagarin Avenue, 23, Nizhny Novgorod, 603950, Russia.; Izquierdo MA; Department of Chemistry, Imperial College London, South Kensington, London SW7 2AZ, UK.; López-Duarte I; Department of Chemistry, Imperial College London, South Kensington, London SW7 2AZ, UK.; Bull JA; Department of Chemistry, Imperial College London, South Kensington, London SW7 2AZ, UK.; Shirmanova MV; Institute of Biomedical Technologies, Nizhny Novgorod State Medical Academy, Minin and Pozharsky Square, 10/1, Nizhny Novgorod, 603005, Russia.; Klapshina LG; Razuvaev Institute of Organometallic Chemistry RAS, Tropinina Street, 49, Nizhny Novgorod, 603950, Russia.; Zagaynova EV; Institute of Biomedical Technologies, Nizhny Novgorod State Medical Academy, Minin and Pozharsky Square, 10/1, Nizhny Novgorod, 603005, Russia.; Kuimova MK; Department of Chemistry, Imperial College London, South Kensington, London SW7 2AZ, UK.
Source
Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
Subject
Language
English
Abstract
The microscopic viscosity plays an essential role in cellular biophysics by controlling the rates of diffusion and bimolecular reactions within the cell interior. While several approaches have emerged that have allowed the measurement of viscosity and diffusion on a single cell level in vitro, the in vivo viscosity monitoring has not yet been realized. Here we report the use of fluorescent molecular rotors in combination with Fluorescence Lifetime Imaging Microscopy (FLIM) to image microscopic viscosity in vivo, both on a single cell level and in connecting tissues of subcutaneous tumors in mice. We find that viscosities recorded from single tumor cells in vivo correlate well with the in vitro values from the same cancer cell line. Importantly, our new method allows both imaging and dynamic monitoring of viscosity changes in real time in live animals and thus it is particularly suitable for diagnostics and monitoring of the progress of treatments that might be accompanied by changes in microscopic viscosity.
Competing Interests: The authors declare no competing financial interests.
Competing Interests: The authors declare no competing financial interests.