학술논문
RNA-sequence analysis of gene expression from honeybees (Apis mellifera) infected with Nosema ceranae.
Document Type
Article
Author
Source
Subject
*HONEYBEE genetics
*NOSEMA ceranae
*NUCLEOTIDE sequence
*GENETIC transcription
*GENE expression
*INSECT genetics
*INSECTS
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Language
ISSN
1932-6203
Abstract
Honeybees (Apis mellifera) are constantly subjected to many biotic stressors including parasites. This study examined honeybees infected with Nosema ceranae (N. ceranae). N. ceranae infection increases the bees energy requirements and may contribute to their decreased survival. RNA-seq was used to investigate gene expression at days 5, 10 and 15 Post Infection (P.I) with N. ceranae. The expression levels of genes, isoforms, alternative transcription start sites (TSS) and differential promoter usage revealed a complex pattern of transcriptional and post-transcriptional gene regulation suggesting that bees use a range of tactics to cope with the stress of N. ceranae infection. N. ceranae infection may cause reduced immune function in the bees by: (i)disturbing the host amino acids metabolism (ii) down-regulating expression of antimicrobial peptides (iii) down-regulation of cuticle coatings and (iv) down-regulation of odorant binding proteins. [ABSTRACT FROM AUTHOR]