학술논문
The Impact of Different Cell Culture Mediums on CD8+ T Cells Expansion: A Bioinformatics Study.
Document Type
Article
Source
Subject
*TELOMERES
*CELL culture
*T cells
*GENE expression profiling
*LIPID synthesis
*CELLULAR aging
*BIOINFORMATICS
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Language
ISSN
2228-5806
Abstract
Objective: Different Cell Culture medias can affect the expansion of T cells. The aim of this study is to assess signaling pathways, protein interactions and genes in T cells cultured in different common T cell expansion medias to select the best candidate. MaterialsAndMethods: In this in silico observational study, with the use of bioinformatics analysis and the use of enrichment databases, gene expression profiles were investigated using microarray analysis. Results: The results of this study were the joint selection of 26 upregulated genes and 59 downregulated genes that were involved in SREBP control of lipid synthesis, co-stimulatory signal during T-cell activation mitosis and chromosome dynamics, telomeres, telomerase, and cellular aging signal pathways. Conclusion: Using bioinformatics analyzes, integrated and regular genes were selected as common genes CD80, LST1, ATM and ITM2B 4-1BBL, Akt inhibitor, interleukin 7 and 15 expansion media. [ABSTRACT FROM AUTHOR]