부산대학교 도서관

Background: Testicular torsion/detorsion can lead to severe testicular damage. The organ‐protective effect of remote ischemic postconditioning (RLIPost) against ischemia/reperfusion injury has been characterized; however, it remains unknown whether RLIPost has a testicular protective effect. Objectives: Here, we tested the hypothesis that RLIPost can protect the testes in a rat model of testicular torsion/detorsion in vivo. Materials and methods: Male Sprague–Dawley rats were assigned to sham‐operated, control, or remote liver and limb ischemic postconditioning‐treated groups. Testicular torsion/detorsion was performed by 3 h of testicular torsion (720° clockwise unilateral spermatic cord torsion), followed by 3 h of detorsion. For liver and limb ischemic postconditioning, four cycles of 5 min of liver or limb ischemia with 5‐min intermittent reperfusion stimuli were conducted at the onset of testicular reperfusion. Results: Liver and limb ischemic postconditioning significantly ameliorated ipsilateral and contralateral testicular swelling responses, preserved morphological integrity and spermatogenesis and inhibited testicular apoptosis. In addition, RLIPost enhanced the phosphorylation of AKT/ERK1/2/GSK‐3β/STAT‐3 in the ipsilateral testis while suppressing JNK activation in the ipsilateral and contralateral testes. Discussion and conclusion: To the best of our knowledge, this study is the first to demonstrate the involvement of RLIPost in an animal model of testicular torsion/detorsion. We showed that RLIPost protects both ipsilateral and contralateral testes against testicular torsion/detorsion in vivo, via at least in part, the RISK and SAFE‐mediated signaling pathways. [ABSTRACT FROM AUTHOR]