학술논문
Cytogenetic Characterization and Cell Cycle Analysis of Three Human Colon Adenocarcinoma Cell Lines: Comparison Between Two- and Three-Dimensional Cell Culture Systems.
Document Type
Article
Author
Source
Subject
*CYTOGENETICS
*CELL cycle
*ADENOCARCINOMA
*MORPHOLOGY
*KARYOTYPES
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Language
ISSN
0735-7907
Abstract
The aim of the study was to investigate whether changes in the pattern of gene copy number and cell cycle were present passing from the two- to the three-dimensional cell culture system. We used three human colon adenocarcinoma cell lines grown two- and three-dimensionally. We analyzed morphology, karyotype, chromosomal gain and losses, and cell cycle. In three-dimensional cell cultures the growth is delayed and arrested in G1 phase without specific rearrangements in the three-dimensional cultures compared to the two-dimensional cultures. These data suggest that the differences between the two- and three-dimensional cell culture systems do not involve chromosomal rearrangements. [ABSTRACT FROM AUTHOR]